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CONTACT USPolymerase chain reaction (PCR) is one of the widely used amplification techniques due to its high sensitivity and good reproducibility. However, the amplified DNA fragments (referred to as 'amplicons'), which serve as templates for further rounds of amplification, can become a source of contamination in the clinical laboratory environment [1]. To prevent the occurrence of false-positive reactions and to ensure the accuracy of PCR results, special measures should be taken to control contamination. Substituting dUTP for dTTP during PCR and treating subsequent reactions with uracil-DNA glycosylase (UDG) prior to amplification is a common strategy to limit carryover contamination [2].
| Recombinant | Expressed in E. coli |
| Form | Solution |
| Concentration | 1 U/μL |
| Inactivation | Heat the mixture to 50 °C for 10 min. |
| Shipped in | Dry ice |
| Storage Condition | Store at -20 °C. Please avoid freeze-thaw cycles. |
| Note | This product is for research use only. |
UDG is mainly used to eliminate the contamination problems posed by the PCR amplification process and its mechanism of action is mainly as follows [3]: UDG cleaves uracil bases from the phosphodiester backbone of uracil-containing DNA, but has no effect on natural (i.e., thymine-containing) DNA. The resulting apyrimidinic sites hinder DNA polymerase replication, and are very sensitive to acid/base hydrolysis. Since UDG does not react with dUTP and is also inactivated by thermal denaturation prior to the actual PCR, PCR carryover contamination can be effectively controlled if the contaminant contains uracil rather than thymine.
Since the SARS-CoV-2 pandemic, research into the detection and treatment of Coronavirus has never stopped. Alfa Chemistry is committed to providing customers with UDG used in the nucleic acid detection process to meet their various needs. If the product you are looking for is not in our catalog, please contact us. We will be happy to provide you with customized synthesis services.
Our products and services are for research use only and cannot be used for any clinical purposes.
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