Vaccine Lab / Alfa Chemistry
Uracil-DNA Glycosylase (UDG)
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Uracil-DNA Glycosylase (UDG)

Polymerase chain reaction (PCR) is one of the widely used amplification techniques due to its high sensitivity and good reproducibility. However, the amplified DNA fragments (referred to as 'amplicons'), which serve as templates for further rounds of amplification, can become a source of contamination in the clinical laboratory environment [1]. To prevent the occurrence of false-positive reactions and to ensure the accuracy of PCR results, special measures should be taken to control contamination. Substituting dUTP for dTTP during PCR and treating subsequent reactions with uracil-DNA glycosylase (UDG) prior to amplification is a common strategy to limit carryover contamination [2].

Uracil-DNA Glycosylase (UDG)

Product Details

RecombinantExpressed in E. coli
Concentration1 U/μL
InactivationHeat the mixture to 50 °C for 10 min.
Shipped inDry ice
Storage ConditionStore at -20 °C. Please avoid freeze-thaw cycles.
NoteThis product is for research use only.

Product Action Mechanism

UDG is mainly used to eliminate the contamination problems posed by the PCR amplification process and its mechanism of action is mainly as follows [3]: UDG cleaves uracil bases from the phosphodiester backbone of uracil-containing DNA, but has no effect on natural (i.e., thymine-containing) DNA. The resulting apyrimidinic sites hinder DNA polymerase replication, and are very sensitive to acid/base hydrolysis. Since UDG does not react with dUTP and is also inactivated by thermal denaturation prior to the actual PCR, PCR carryover contamination can be effectively controlled if the contaminant contains uracil rather than thymine.

Product Applications

Product Applications

Since the SARS-CoV-2 pandemic, research into the detection and treatment of Coronavirus has never stopped. Alfa Chemistry is committed to providing customers with UDG used in the nucleic acid detection process to meet their various needs. If the product you are looking for is not in our catalog, please contact us. We will be happy to provide you with customized synthesis services.


  1. Pang, J.; et al. Use of modified nucleotides and uracil-DNA glycosylase (UNG) for the control of contamination in the PCR-based amplification of RNA. Molecular and cellular probes. 1992, 6(3): 251-256.
  2. Pierce, K. E.; et al. Effectiveness and limitations of uracil-DNA glycosylases in sensitive real-time PCR assays. Biotechniques. 2004, 36(1): 44-48.
  3. Longo, M. C.; et al. Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene. 1990, 93(1): 125-128.

Our products and services are for research use only and cannot be used for any clinical purposes.

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