Phi29 DNA polymerase is an enzyme from the bacteriophage phi29. It has found increasing use in molecular biology. Phi29 DNA polymerase has two unique properties. Firstly, it has a sustained synthesis capacity, enabling the enzyme to perform strand displacement DNA synthesis of over 70,000 nt without dissociating from the template, and its stability allows efficient DNA synthesis to continue for several hours . In addition, it has strand displacement activity, enabling the enzyme to replace the complementary strand in the double-stranded region of the template molecule during DNA synthesis . Because of these two special properties of phi29 DNA polymerase, the elongating stage of phi29 DNA replication requires neither accessory protein nor helicase. From an application point of view, these two important properties of phi29 DNA polymerase, together with its high fidelity to DNA synthesis, constitute the basis for the application of this enzyme in an increasing number of isothermal DNA amplification procedures in vitro .
Figure 1. Replication diagram of DNA.
|Unit Size||1000 U|
|Incubation||Incubate at 30 °C.|
|Inactivation||Heat the mixture to 65 °C for 15 min.|
|Storage Condition||Store at -20 °C. Please avoid freeze-thaw cycles.|
|Note||This product is for research use only.|
- Routine PCR
- High throughput PCR
- Multiplex PCR
- Rolling circle amplification (RCA)
- Multiple Displacement Amplification (MDA)
- Whole Genome Amplification (WGA)
- Preparation of DNA template for sequencing
- Amplification of DNA from filter paper blood spot samples
- Protein-primed DNA amplification
- In situ genotyping with padlock probes
- Recombination based-cloning
- RNA-primed DNA amplification
Since the SARS-CoV-2 pandemic, research into the detection and treatment of Coronavirus has never stopped. Alfa Chemistry is committed to providing customers with the phi29 DNA polymerase used in the nucleic acid detection process to meet their various needs. If the product you are looking for is not in our catalog, please contact us. We will be happy to provide you with customized synthesis services.
- Dean, F. B.; et al. Rapid amplification of plasmid and phage DNA using phi29 DNA polymerase and multiply-primed rolling circle amplification. Genome research. 2001, 11(6): 1095-1099.
- Johne, R.; et al. Rolling-circle amplification of viral DNA genomes using phi29 polymerase. Trends in microbiology. 2009, 17(5): 205-211.
- Salas, M.; et al. Phi29 DNA polymerase, a potent amplification enzyme. 2004.
Our products and services are for research use only and cannot be used for any clinical purposes.