Phi29 DNA Polymerase

Phi29 DNA polymerase is an enzyme from the bacteriophage phi29. It has found increasing use in molecular biology. Phi29 DNA polymerase has two unique properties. Firstly, it has a sustained synthesis capacity, enabling the enzyme to perform strand displacement DNA synthesis of over 70,000 nt without dissociating from the template, and its stability allows efficient DNA synthesis to continue for several hours ^[1]. In addition, it has strand displacement activity, enabling the enzyme to replace the complementary strand in the double-stranded region of the template molecule during DNA synthesis ^[2]. Because of these two special properties of phi29 DNA polymerase, the elongating stage of phi29 DNA replication requires neither accessory protein nor helicase. From an application point of view, these two important properties of phi29 DNA polymerase, together with its high fidelity to DNA synthesis, constitute the basis for the application of this enzyme in an increasing number of isothermal DNA amplification procedures in vitro ^[3].

Figure 1. Replication diagram of DNA.

Since the SARS-CoV-2 pandemic, research into the detection and treatment of Coronavirus has never stopped. Alfa Chemistry is committed to providing customers with the phi29 DNA polymerase used in the nucleic acid detection process to meet their various needs. If the product you are looking for is not in our catalog, please contact us. We will be happy to provide you with customized synthesis services.

References

1. Dean, F. B.; et al. Rapid amplification of plasmid and phage DNA using phi29 DNA polymerase and multiply-primed rolling circle amplification. Genome research. 2001, 11(6): 1095-1099.
2. Johne, R.; et al. Rolling-circle amplification of viral DNA genomes using phi29 polymerase. Trends in microbiology. 2009, 17(5): 205-211.
3. Salas, M.; et al. Phi29 DNA polymerase, a potent amplification enzyme. 2004.

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Phi29 DNA Polymerase

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