Deoxyribonuclease I (usually called DNase I), originally discovered from bovine pancreas, is a recombinant enzyme expressed in Pichia pastoris. It is a specific endonuclease that acts on single-stranded DNA, double-stranded DNA and chromatin and especially shows a strong preference for double-stranded substrates. It preferentially cleaves DNA at phosphodiester linkages adjacent to a pyrimidine nucleotide, producing tri- and/or tetra-oligonucleotides with 5'-phosphate and 3'-hydroxyl termini. To obtain full enzymatic activity, the pH is approximately 7.5, and micromolar concentrations of Ca2+, Mg2+, or Mn2+ are required. Its molecular mass is around 31-34 KDa depending on the amino acid composition and the extent of glycosylation, both varying in a tissue- and species-specific manner .
|Recombinant||Expressed in Pichia pastoris|
|Activators||DNase I requires divalent cations to obtain maximum activity.|
|Inhibitors||EDTA, EGTA, SDS|
|Inactivation||Heat the mixture to 75 °C for 10 min.|
|Shipped in||Dry ice|
|Storage Temperature||2-8 °C|
|Note||This product is for research use only.|
- Removal of template DNA after in vitro transcription
- Removal of contaminating genomic DNA from RNA samples
- DNA labeling by nick-translation
- Preparation of DNA-free RNA before RT-qPCR and RT-PCR
- Footprinting analysis
- Microarray analysis
- Chromatin structure analysis
- Molecular cloning and DNA sequencing
It is important to note that the enzyme needs to function under appropriate conditions, such as:
- 1X DNase I Reaction Buffer
- Incubation at 37 °C
Since the SARS-CoV-2 pandemic, research into the detection and treatment of Coronavirus has never stopped. Alfa Chemistry is committed to providing customers with DNase I used in the nucleic acid detection process to meet their various needs. If the product you are looking for is not in our catalog, please contact us. We will be happy to provide you with customized synthesis services.
- Valle, F. M.; et al. DNase 1 and systemic lupus erythematosus. Autoimmunity reviews. 2008, 7(5): 359-363.
Our products and services are for research use only and cannot be used for any clinical purposes.