Vaccine Lab / Alfa Chemistry
Nitrocellulose Membranes
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Nitrocellulose Membranes

Nitrocellulose (NC) membranes are a series of paper-like matrices with micro-scale porous structures. Due to their good biocompatibility, unique physical and chemical properties, and the ability to immobilize various biomolecules, NC membranes have been widely used in the biomedical field. For example, NC membranes are commonly used to immobilize nucleic acids in southern blots and northern blots, and to immobilize proteins in western blots [1]. In addition, they are also considered to be the backbone of the rapid test strip, and the capture antibodies of the test line and the control line are coated here [2].

Nitrocellulose Membranes

Product Details

AppearanceHigh quality film with flat and smooth surface
Pore Size0.2µm: Transfer of low molecular weight proteins (<20 kDa) and nucleic acids (<300 bp).
0.45 µm: Transfer of most proteins (>20 kDa) and nucleic acids (>300 bp).
Thickness230 ± 20 μm
Filter MediaDense 100% nitrocellulose
SensitivityWeak to strong
C Line Visible SpeedFast to slow
Binding InteractionHydrophobic and electrostatic
Applications(i) Western, northern and southern transfers
(ii) Immunoassay of target protein
(iii) Probe hybridization of nucleic acids
(iv) Dot or slot blotting

Product Features

  • NC membranes are compatible with all standard immunoblotting and nucleic acid detection methods.
  • NC membranes are composed of 100% dense nitrocellulose to provide high quality transfer.
  • NC membranes provide excellent binding ability without background interference.
  • NC membranes are available in three supply formats:
    (i) Convenient precut membrane/filter paper sandwich
    (ii) Packs of precut membrane-only sheets
    (iii) Economical rolls

Since the SARS-CoV-2 pandemic, research into the detection and treatment of Coronavirus has never stopped. Alfa Chemistry is committed to providing customers with NC membranes to meet their various needs. If the product you are looking for is not in our catalog, please contact us. We will be happy to provide you with customized synthesis services.


  1. Sun, S.; et al. Microstructural effects on permeability of Nitrocellulose membranes for biomedical applications. Journal of Membrane Science. 2020, 595: 117502.
  2. Sukumaran, A.; et al. Development and troubleshooting in lateral flow immunochromatography assays. Indian Journal of Clinical Biochemistry. 2021, 36(2): 208-212.

Our products and services are for research use only and cannot be used for any clinical purposes.

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