Nitrocellulose (NC) membranes are a series of paper-like matrices with micro-scale porous structures. Due to their good biocompatibility, unique physical and chemical properties, and the ability to immobilize various biomolecules, NC membranes have been widely used in the biomedical field. For example, NC membranes are commonly used to immobilize nucleic acids in southern blots and northern blots, and to immobilize proteins in western blots . In addition, they are also considered to be the backbone of the rapid test strip, and the capture antibodies of the test line and the control line are coated here .
|Appearance||High quality film with flat and smooth surface|
|Pore Size||0.2µm: Transfer of low molecular weight proteins (＜20 kDa) and nucleic acids (＜300 bp). |
0.45 µm: Transfer of most proteins (＞20 kDa) and nucleic acids (＞300 bp).
|Thickness||230 ± 20 μm|
|Filter Media||Dense 100% nitrocellulose|
|Sensitivity||Weak to strong|
|C Line Visible Speed||Fast to slow|
|Binding Interaction||Hydrophobic and electrostatic|
|Applications||(i) Western, northern and southern transfers|
(ii) Immunoassay of target protein
(iii) Probe hybridization of nucleic acids
(iv) Dot or slot blotting
- NC membranes are compatible with all standard immunoblotting and nucleic acid detection methods.
- NC membranes are composed of 100% dense nitrocellulose to provide high quality transfer.
- NC membranes provide excellent binding ability without background interference.
- NC membranes are available in three supply formats:
(i) Convenient precut membrane/filter paper sandwich
(ii) Packs of precut membrane-only sheets
(iii) Economical rolls
Since the SARS-CoV-2 pandemic, research into the detection and treatment of Coronavirus has never stopped. Alfa Chemistry is committed to providing customers with NC membranes to meet their various needs. If the product you are looking for is not in our catalog, please contact us. We will be happy to provide you with customized synthesis services.
- Sun, S.; et al. Microstructural effects on permeability of Nitrocellulose membranes for biomedical applications. Journal of Membrane Science. 2020, 595: 117502.
- Sukumaran, A.; et al. Development and troubleshooting in lateral flow immunochromatography assays. Indian Journal of Clinical Biochemistry. 2021, 36(2): 208-212.
Our products and services are for research use only and cannot be used for any clinical purposes.