Proteinase K

Product Name	Proteinase K
CAS Number	39450-01-6
Molecular Weight	28.9 kDa
Appearance	Lyophilized powder
Specific Activity	≥30 units/mg protein
Storage Condition	Store at -20 °C
Note	This product is for research use only.

Our Proteinase K remains stable over a wide pH range (optimum between 6.5-9.5) and relatively high temperatures (optimum between 50-65 °C).
Our Proteinase K is active in the presence of protein denaturing chemicals such as SDS and urea, chelating agents such as EDTA, hydrogen sulfide reagents and trypsin or chymotrypsin inhibitors.
Our Proteinase K is DNase free, RNase free and has no nucleic acid residues.

Proteinase K activity in commonly used buffers is shown in the following table ^[2].

Buffer (pH = 8.0, 50 °C, 1.25 µg/mL protease K, 15 min incubation)	Proteinase K activity
30 mM Tris·Cl	100%
30 mM Tris·Cl; 30 mM EDTA; 5% Tween 20; 0.5% Triton X-100; 800 mM GuHCl	313%
36 mM Tris·Cl; 36 mM EDTA; 5% Tween 20; 0.36% Triton X-100; 735 mM GuHCl	301%
10 mM Tris·Cl; 25 mM EDTA; 100 mM NaCl; 0.5% SDS	128%
10 mM Tris·Cl; 100 mM EDTA; 20 mM NaCl; 1% Sarkosyl	74%
10 mM Tris·Cl; 50 mM KCl; 1.5 mM MgCl2; 0.45% Tween 20; 0.5% Triton X-100	106%
10 mM Tris·Cl; 100 mM EDTA; 0.5% SDS	120%
30 mM Tris·Cl; 10 mM EDTA; 1% SDS	203%

Our Proteinase K is commonly used in molecular biology to digest proteins and remove contaminants from nucleic acid preparations.
Our Protease K is used in the separation of plasmids, genomic DNA or RNA.
Our proteinase K can inactivate RNases, DNases and enzymes in reactions.
Our proteinase K is used for the destruction of proteins in cell lysates (tissues, cell culture cells) and for the release of nucleic acids.
Our Proteinase K can analyze membrane structures by modifying proteins and glycoproteins on the cell surface.
Our Proteinase K can treat tissue sections to ensure effective infiltration of the probe during in situ hydridization.