Zhu S-C, et al. Journal of Chromatography A, 2022, 1678, 463356.
Sodium chenodeoxycholate (Na-CDC), a bile salt-derived biosurfactant, has gained significant attention in recent years for its potential in enhancing extraction methods in phytochemical analysis. Its amphiphilic structure allows it to effectively interact with both hydrophilic and hydrophobic compounds, making it a versatile agent in the extraction of diverse bioactive constituents from complex matrices.
A recent study demonstrated the successful application of Na-CDC in a biosurfactant-assisted mechanical amorphous dispersion extraction (BA-MADE) method for Salvia miltiorrhiza samples. Under the optimized conditions, 407.02 mg of Na-CDC, a grinding time of 4.87 minutes, an extraction time of 4.92 minutes, and a solid-to-liquid ratio of 0.5:10 g/mL were found to yield superior extraction results. The method showed high precision and accuracy, with limits of detection ranging from 5.46 to 130 ng/mL and recoveries of the real sample ranging from 85% to 113%. In comparison with conventional extraction methods, such as heating reflux extraction, ultrasonic extraction, and microwave-assisted micellar extraction, the BA-MADE method demonstrated significant advantages in terms of efficiency and simultaneous extraction of hydrophilic and hydrophobic compounds.