CAS 849110-74-3 N-dodecyl-D25-β-D-maltopyranoside

Characterization of N-Dodecyl-β-D-Maltopyranoside in Intramembrane Aspartyl Proteases Research

Solution Structure of an Intramembrane Aspartyl Protease via Small Angle Neutron Scattering Naing S-H, et al. Biophysical Journal, 2018, 114(3), 602-608.

Intramembrane aspartyl proteases (IAPs) are integral membrane proteases that hydrolyze substrates within the hydrophobic lipid bilayer. This case study investigates the use of n-dodecyl-β-D-maltopyranoside (DDM) in the characterization of IAPs, focusing on their oligomerization state in solution using small-angle neutron scattering (SANS).
Methods:
To investigate the oligomerization state of IAPs, SANS was employed to characterize detergent solutions of DDM with and without a microbial IAP ortholog. SANS allows modulation of the solvent composition to isolate the signal from the enzyme of interest. By using deuterated DDM and D₂O, scattering from the detergent and buffers was matched, enhancing the signal from the IAP.
Results:
The radius of gyration calculated for the IAP, along with the corresponding ab initio consensus model, indicates that the protein exists as a monomer in solution. This model appears slightly smaller than the crystallographic IAP monomer, suggesting a more compact structure in solution compared to the crystal lattice.
Conclusion:
The study provides direct insight into the oligomeric state of purified IAPs in surfactant solution, demonstrating that the IAP exists as a monomer. The use of fully contrast-matched DDM in SANS proved effective in characterizing the IAP, showcasing the potential of this technique for studying other intramembrane proteases and their membrane-bound substrates.
DDM effectively facilitated the characterization of IAPs using SANS, revealing a monomeric state in solution. This approach highlights the utility of SANS with contrast-matched detergents for investigating the structure-function relationships of membrane-bound proteins.

Catalog Number	ACM849110743
CAS	849110-74-3
Molecular Weight	510.62
Molecular Formula	C24H46O11
Purity	97%