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CONTACT US| Catalog Number | ACMA00024677 |
| Description | One end of the PEG of this product is connected to DSPE, and the other end is connected to creka. Through creka coupling, the fibrin binding capacity of PEG particles can be increased to 94%. |
| Purity | 90-97% |
| Solubility | Soluble in chloroform, methanol, DMSO |
| Application | Targeting the cellular microenvironment via nano-particle-based therapies holds great promise for the treatment of various diseases. |
| One-Letter Symbol Of Polypeptide | CREKA |
| Physical State | White/Off-white solid or viscous liquid |
| Storage Conditions | Stored at -20 °C, protected from light and moisture |
| Substitution By NMR | >95% |
| Three-Letter Symbol Of Polypeptide | Cys-Arg-Glu-Lys-Ala |
DSPE-PEG, short for 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)], is a synthetic phospholipid that is widely utilized in various pharmaceutical and biomedical applications due to its unique properties. Get more information on:
Wang C, et al. International Journal of Nanomedicine, 2015, 10(1), 2229-2248.
DSPE-PEG-CREKA was used to prepare a novel targeted heat-sensitive liposome, CREKA-modified LTSL (DOX-LTSL-CREKA) containing adriamycin (DOX). The CREKA peptide recognizes coagulated plasma proteins in tumor vasculature and stroma, and the heat-sensitive liposome immediately releases the encapsulated drug in heated tumor vessels.
Preparation of DOX-LTSL-CREKA
DOX-LTSL-CREKA were prepared using a thin-film hydration method as described previously. DPPC, MSPC, DSPE-PEG, and DSPE-PEG-CREKA (in an 86:10:2:2 molar ratio) were dissolved in chloroform. After evaporating the solvent in an RE52 rotary evaporator in a round-bottomed flask at 45°C for about 40 minutes, a solid film formed. This film was then flushed with nitrogen gas for 30 minutes and stored overnight in a desiccator to remove any traces of chloroform.
Next, 2 mL of 300 mM citric acid buffer (pH 4.0) was added, and the mixture was sonicated in a bath sonicator for 30 minutes. The resulting liposomes were then extruded eleven times through 100 nm polycarbonate membrane filters using a mini-extruder. The obtained liposomes were then passed through a Sephadex G-50 column to remove the external citric acid using a 20 mM Hepes buffer solution (HBS, pH 7.4) containing 150 mM NaCl to obtain the blank liposomes. DOX was loaded into these blank liposomes using a previously described pH-gradient method. An aqueous DOX solution was added to the blank liposomes at a DOX/lipid (w/w) ratio of 1:20. The mixed solution was incubated for 1 hour at 37°C and then passed through a Sephadex G-50 column to remove the unentrapped DOX with HBS.
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