Deae-cellulose

Catalog Number	ACM9004346-37
CAS	9004-34-6
Structure
Synonyms	Microcrystalline cellulose
IUPAC Name	(6S)-2-(Hydroxymethyl)-6-[(3S)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxane-3,4,5-triol
Molecular Weight	342.3
Molecular Formula	C12H22O11
Canonical SMILES	C(C1C(C(C(C(O1)OC2C(OC(C(C2O)O)O)CO)O)O)O)O
InChI	InChI=1S/C12H22O11/c13-1-3-5(15)6(16)9(19)12(22-3)23-10-4(2-14)21-11(20)8(18)7(10)17/h3-20H,1-2H2/t3,4,5,6,7,8,9,10-,11,12+/m1/s1
InChI Key	GUBGYTABKSRVRQ-WFVLMXAXSA-N
Melting Point	76-78 °C
Purity	98%
Complexity	382
Covalently-Bonded Unit Count	1
Defined Atom Stereocenter Count	2
Exact Mass	342.11621151
Heavy Atom Count	23
Hydrogen Bond Acceptor Count	11
Hydrogen Bond Donor Count	8
Isomeric SMILES	C(C1[C@H](C(C(C(O1)O)O)O)O[C@H]2C(C(C(C(O2)CO)O)O)O)O
Monoisotopic Mass	342.11621151
Physical State	Powder
Rotatable Bond Count	4
Topological Polar Surface Area	190 Ų

Knowledge & Learning Case Study Q&A

DEAE-cellulose as Adsorbent for Removal of 2,4-Dichlorophenoxyacetic Acid

Kodali J, et al. RSC Adv, 2021, 11, 22640-22651.

2,4-Dichlorophenoxyacetic acid (2,4-D) is a common herbicide used in agriculture. However, its presence in water and agricultural soil runoff poses significant environmental and health risks. DEAE-cellulose is explored as a potential adsorbent to remove (2,4-D) from contaminated water and soil runoff to address this issue.
Material Characterization:
The DEAE-cellulose is characterized using scanning electron microscopy (SEM) before and after the adsorption of 2,4-D. The SEM images revealed that the surface of DEAE-cellulose became smoother after 2,4-D adsorption, with rough patches covered in dark spots, indicating the presence of the herbicide.
To confirm the elemental composition of 2,4-D on DEAE-cellulose, energy-dispersive X-ray spectroscopy (EDX) is employed. The EDX spectra showed significant peaks for chlorine and other elements, verifying the adsorption of 2,4-D on the DEAE-cellulose surface.
Adsorption Efficiency: The adsorption of 2,4-D on DEAE-cellulose is found to be highly effective at neutral pH. The Langmuir maximum adsorption capacity is determined to be 429 mg/g, indicating a high affinity of DEAE-cellulose for 2,4-D.
Thermodynamics and Kinetics: The adsorption process is characterized by favorable thermodynamics, showing spontaneous adsorption with exothermic enthalpy. The kinetics of adsorption followed a pseudo-second-order model, indicating rapid adsorption rates.
Mechanistic Insights: Comprehensive analysis revealed that the adsorption mechanism involved electrostatic interactions, hydrogen bonding, and Lewis acid-base interactions. These interactions contributed to the high efficiency of DEAE-cellulose in removing 2,4-D from aqueous solutions.
Through this research, DEAE-cellulose has proven to be a significant and effective material for the removal of 2,4-D, contributing to a safer and cleaner environment.

DEAE-cellulose as a Control for Resin Purification of Trypanosomes

Marques J, et al. Analytical Biochemistry, 2023, 676, 115226.

DEAE-celluloseis renowned for its efficiency in purifying blood samples containing trypanosomes. The resin's weakly basic structure, derived from tertiary amine functional groups linked to a hydrophilic matrix, facilitates the isolation of live parasites.
Experimental Design
A comparative study is conducted to evaluate the efficacy of DEAE-cellulose against three other resins: PURIFICA Y-C2N, Y-HONOH, and Y-CNC3, for purifying Trypanosoma evansi. DEAE-cellulose served as the control due to its established purification capabilities.
T. evansi Isolation and Purification
The ion exchange chromatography method is employed to isolate T. evansi using DEAE-cellulose and PURIFICA resins. Whole blood is homogenized with Percoll and centrifuged. The homogenized sample is further processed in PBS-G, with subsequent centrifugation steps to concentrate the parasites. Post-ishing, the parasites are quantified using a Neubauer chamber.
Morphological Examination
The morphology of T. evansi is examined pre- and post-purification using optical microscopy at 100x magnification. The parasites are stained using the Panotype Rapid kit and assessed for characteristic features such as the thin posterior end, free flagellum, and undulating membrane to ensure morphological integrity.
The study concluded that PURIFICA Y-C2N, Y-HONOH, and Y-CNC3 resins are viable, cost-effective alternatives to DEAE-cellulose for purifying T. evansi in developing countries.

DEAE-cellulose for the Preparation of Multicomponent Biomaterial Inks

Vel R, et al. Materials Today Communications, 2022, 33, 104335.

In this case, alginate (ALG, to provide stability), gelatin (GEL, to enhance cell adhesion), and diethylaminoethyl cellulose (DEAE-Cellulose, for viscosity modulation/shear thinning) are integrated to prepare multi-component biomaterial inks (ALG/GEL/DEAE-Cellulose) for extrusion-based 3D printing. The formulations are prepared as follows:
First, 15% (w/v) of the raw gelatin material is prepared, and the desired amount of GEL powder is completely dissolved in DMEM-F12 medium and stirred continuously at 37℃ using a magnetic stirrer at 100 rpm. Different concentrations (1-2%) of alginate are prepared by dissolving the desired amount (w/v) in Hank's balanced salt solution (HBSS) and stirring continuously overnight at 100 rpm to make a homogeneous and viscous solution, and then DEAE-cellulose powders are added to achieve the final concentrations of 0.45%, 0.9%, and 2% (w/v) with continuous 200 rpm stirring for 45 minutes. Then, the desired volume of 15% gelatin is added to the mixture to achieve a final concentration of 3.3% and stirred at 200 rpm for 30 minutes to obtain a homogeneous formulation. Finally, the formulation is centrifuged at 500 rpm for 5 minutes to remove air bubbles and stored at 37℃ for later use.

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