CAS 63988-21-6 1',3'-Bis[1,2-dimyristoyl-sn-glycero-3-phospho]-glycerol (sodium salt)

1',3'-Bis[1,2-Dimyristoyl-sn-Glycero-3-Phospho]-Glycerol for the Preparation of Binary Mixtures for Lipid Membrane Studies

A model membrane composed of a binary mixture of 1',3'-Bis[1,2-dimyristoyl-sn-glycero-3-phospho]-glycerol (TMCL) and the zwitterionic phospholipid dimyristoylphosphatidylethanolamine (DMPE). Benesch MGK, et al. Biochim Biophys Acta, 2015, 1848: 2878-2888.

The structure and organization of lipid bilayer model membranes composed of mixtures of two or more lipids are of particular biological importance. In this case study, a model membrane composed of a binary mixture of 1',3'-Bis[1,2-dimyristoyl-sn-glycero-3-phospho]-glycerol (TMCL) and the zwitterionic phospholipid dimyristoylphosphatidylethanolamine (DMPE) is investigated.
The binary lipid mixtures are prepared by combining chloroform:methanol (9:1) solutions of the lipid components in the required proportions to obtain the desired composition. The solvent is then slowly removed under a stream of nitrogen gas, allowing the lipid mixture to be cast as a thin film on the side of a clean glass tube while maintaining the temperature close to 60 ℃. The lipid film is then dried overnight under vacuum to ensure complete removal of the last traces of solvent.
Hydration of the lipid film is achieved by heating the sample to approximately 60 ℃. This is done by placing some wet cotton wool in the tube (without allowing it to touch the lipid film), which allowed the sample to absorb water vapor from the saturated air. After the hydration step, the cotton wool is removed and the hydrated sample is quickly dispersed in a pre-warmed buffer solution. The buffer consists of 100 mM phosphate, 5 mM sodium azide (NaN₃), 150 mM sodium chloride (NaCl), at pH 7.4. The sample is mixed thoroughly by vigorous vortexing at a temperature close to 60 ℃.

Catalog Number	ACM63988216
CAS	63988-21-6
Structure
Description	Cardiolipin, the mitochondrial-specific phospholipid, helps maintain mitochondrial function, membrane potential, and structural support for the inner mitochondrial membrane and the proteins in it. Concentration and composition changes of cardiolipin have been implicated in pathological conditions including ischemia, hypothyroidism, aging, heart failure, and cardio skeletal myopathy (Barth syndrome). Cardiolipin has also been shown as a signaling molecule in apoptosis.
Synonyms	14:0 Cardiolipin (sodium salt), Disodium bis[(2R)-2,3-bis(tetradecanoyloxy)propyl] 2-hydroxy-1,3-propanediyl bis(phosphate), 1,1',2,2'-tetratetradecanoyl cardiolipin (sodium salt); CL(1'-[14:0/14:0],3'-[14:0/14:0])
Molecular Weight	1285.6
Molecular Formula	C65H124Na2O17P2
Purity	>99% (TLC)
Application	14:0 Cardiolipin (sodium salt) has been used for the mass spectrometry (MS) analysis to determine the structure of lipid A.
Composition	C 60.73%, H 9.72%, Na 3.58%, O 21.16%, P 4.82%
Concentration	for solution: 10 mg/mL (powder-25mg) 25 mg/mL (powder-200mg)
Exact Mass	1284.810913
Lipid Type	phospholipids, cardiolipins
MDL Number	MFCD22416743
NACRES	NA.25
Physical State	Powder; Chloroform solution
Storage Conditions	Stored at -20 °C