CAS 4432-31-9 2-(N-Morpholino)-ethanesulfonic acid

Optimization of Capillary Electrophoresis for Separation of Aluminum Chlorohydrates Using 4-Morpholineethanesulfonic Acid

Capillary electrophoresis for aluminum ion speciation: Optimized separation conditions for complex polycation mixtures Ouadah N, et al. Journal of Chromatography A, 2018, 1552, 79-86.

Recent studies have successfully utilized capillary electrophoresis (CE) to achieve the first effective separation and characterization of ACH oligomers, specifically Al13 and Al30, using 4-Morpholineethanesulfonic acid (MES) as the background electrolyte (BGE).
MES plays a crucial role in stabilizing ACH oligomers during electrophoretic separation, ensuring high reproducibility and rapid analysis. The optimization of MES concentration, pH, and ionic strength of the BGE was found to significantly affect the separation process. The primary finding was that ionic strength is the dominant factor influencing resolution, with migration inversions occurring between monovalent (Na+) and multivalent chlorohydrate oligomers due to the dependency of effective mobility on ionic strength.
The study identified optimal separation conditions using 700 mM MES at 25 mM ionic strength and a pH of 4.8, combined with 0.1 mM didodecyldimethylammonium bromide for dynamic capillary coating. Under these conditions, baseline separation of Al13, Al30, Na+, and various aluminum hydroxide colloids and aggregated polycation forms was achieved, demonstrating the method's robustness and applicability to samples that are challenging to analyze using other techniques.

Catalog Number	ACM4432319-1
CAS	4432-31-9
Structure
Synonyms	4-Morpholineethanesulfonic acid
IUPAC Name	2-Morpholin-4-ylethanesulfonic acid
Molecular Weight	195.24
Molecular Formula	C6H13NO4S
Canonical SMILES	C1COCCN1CCS(=O)(=O)O
InChI	InChI=1S/C6H13NO4S/c8-12(9,10)6-3-7-1-4-11-5-2-7/h1-6H2,(H,8,9,10)
InChI Key	SXGZJKUKBWWHRA-UHFFFAOYSA-N
Melting Point	>300 °C
Purity	99%+
Complexity	214
Covalently-Bonded Unit Count	1
Defined Atom Stereocenter Count	0
Exact Mass	195.05652907
Heavy Atom Count	12
Hydrogen Bond Acceptor Count	5
Hydrogen Bond Donor Count	1
Monoisotopic Mass	195.05652907
Physical State	Powder
Rotatable Bond Count	3
Topological Polar Surface Area	75.2 Å²