Product Name: Japanese Encephalitis Virus-Like Particles
Catalog Number: ACVL-VLP004
Category: Virus-Like Particles (VLPs)
Host System: HEK293 (Mammalian)
Components: Recombinant pre-Membrane (prM) and Envelope (E) proteins
Product Overview
Alfa Chemistry provides high-quality Japanese Encephalitis Virus-Like Particles to support vaccine research, immunological studies, and diagnostic assay development. These recombinant particles are produced in HEK293 mammalian cells through co-expression of the viral precursor membrane (prM) and envelope (E) structural proteins derived from the JEV SA-14 strain.
During expression, the structural proteins undergo proper folding and post-translational processing in mammalian cells. As a result, they self-assemble into nanoscale particles that closely resemble native Japanese encephalitis virus virions. Although structurally similar to authentic viruses, these particles lack viral genomic RNA and therefore cannot replicate or cause infection.
Because the envelope proteins are presented in their native conformation and glycosylation context, the resulting VLPs preserve biologically relevant antigenic epitopes. This makes them valuable tools for studies involving immune recognition, neutralizing antibody responses, and antigen-based diagnostic platforms.
Key Technical Features
- Authentic Antigen Presentation: Production in HEK293 mammalian cells supports native-like protein folding and glycosylation, preserving conformational epitopes essential for immune recognition.
- Native Virion-Like Architecture: Co-expression of prM and E proteins enables spontaneous assembly into particles that mimic the structural organization of natural flavivirus virions.
- Non-Infectious Safety Profile: The particles contain no viral genome, making them replication-deficient and safe for laboratory research.
- High-Purity Preparation: Purified from culture supernatant using density-gradient centrifugation and chromatographic techniques to maintain structural integrity and antigenicity.
Background & Biological Significance
Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus and one of the leading causes of viral encephalitis in Asia and the Western Pacific region. Infection can result in severe neurological disease, and survivors may experience long-term neurological complications. Despite the availability of vaccines in some endemic regions, JEV remains a significant public health concern.
JEV contains a positive-sense RNA genome that encodes a single polyprotein, which is subsequently processed into structural and nonstructural proteins. Among these components, the envelope glycoprotein plays a critical role in viral entry by mediating host cell attachment and membrane fusion. It is also the primary target of neutralizing antibodies generated during infection or vaccination.
Virus-like particle technology provides a powerful approach for studying viral antigens without the need for infectious virus. When prM and envelope proteins are co-expressed in suitable host cells, they spontaneously assemble into particles that display viral surface proteins in a native-like arrangement. These characteristics allow VLPs to closely mimic the antigenic structure of the virus while maintaining a strong safety profile.
Applications
Serve as candidate immunogens for evaluating immune responses and developing next-generation flavivirus vaccines.
Suitable as coating antigens for ELISA and other immunoassays used to detect JEV-specific antibodies.
Useful for screening and characterization of monoclonal antibodies targeting JEV envelope proteins.
- Structural and Functional Studies
Support investigations of viral entry mechanisms, antigenic structure, and immune recognition.
Technical Specifications
| Item | Specification |
| Product Name | Japanese Encephalitis Virus-Like Particles |
| Catalog Number | ACVL-VLP004 |
| Expression System | HEK293 mammalian cells |
| Components | prM and Envelope (E) proteins |
| Sequence Reference | NCBI Accession P27395.1 |
| Sequence Region | AA105–819 |
| Strain | SA-14 |
| Formulation | Sterile PBS (pH 7.4) |
Why Choose Alfa Chemistry?
Alfa Chemistry provides high-quality virus-like particle products designed for vaccine research and infectious disease studies. Our VLP production platform integrates mammalian expression systems with advanced purification technologies to ensure structural integrity, reproducibility, and strong antigenicity.
With rigorous quality control and scalable production capabilities, Alfa Chemistry supports researchers and biotechnology developers in accelerating vaccine, diagnostic, and therapeutic development.
Our products and services are for research use only and cannot be used for any clinical purposes.
