Dengue Virus Serotype 1 Virus-Like Particles - Vaccine Lab / Alfa Chemistry

Product Overview

Dengue Virus Serotype 1 (DENV-1) Virus-Like Particles are recombinant nanostructures engineered to replicate the antigenic architecture of native dengue virions while eliminating infectivity. Generated via transient expression in HEK293 cells, these particles assemble spontaneously from viral structural proteins and present conformational epitopes critical for immune recognition.

Unlike inactivated or attenuated viral preparations, these VLPs contain no viral genome, enabling safe handling while maintaining high biological relevance. Their structural fidelity makes them particularly valuable for mechanistic immunology studies and antigen characterization.

Key Technical Features

Authentic Antigen Presentation: The assembled particles display envelope glycoproteins in native conformations, preserving quaternary epitopes essential for antibody binding.

Engineered Secretion Efficiency: Incorporation of Japanese Encephalitis Virus (JEV)-derived sequences within the E protein transmembrane domain enhances particle release and improves yield.

Physiological Maturation Profile: Endogenous furin-mediated cleavage of prM results in a heterogeneous population of partially and fully matures particles, closely mimicking natural dengue virions.

Purification Strategy: Multi-step purification combining ultracentrifugation and chromatography ensures removal of host-derived contaminants.

Biosafety Advantage: Genome-free design eliminates replication capability, supporting use in standard laboratory environments.

Technical Specifications

Item	Specification
Product Name	Dengue Virus Serotype 1 Virus-Like Particles
Catalog Number	ACVL-VLP002
Expression System	HEK293 mammalian cells
Components	Envelope (E), pre-Membrane (prM), and Membrane (M)
Sequence Reference	NCBI Accession ACA48859.1
Formulation	Provided in optimized buffer for maximum stability

Research Applications

Immunogen Screening: Supports evaluation of antigen design strategies for dengue vaccine development.

Serological Assays: Serves as a structurally relevant coating antigen for ELISA and antibody detection platforms.

Functional Antibody Studies: Applicable in neutralization and antibody-dependent enhancement (ADE) investigations.

Structural Characterization: Compatible with cryo-EM and epitope mapping studies.

Product Positioning

This first-generation VLP product is particularly suited for basic research, antigen discovery, and structural analysis, where biological authenticity is the primary requirement.

In addition to Serotype 1, we also provide VLPs for Dengue Serotypes 2, 3, and 4 to support comprehensive multivalent vaccine studies.

Our products and services are for research use only and cannot be used for any clinical purposes.

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